Understanding how tissue damage leading to sprains initiates and propagates is essential for developing adequate prevention and treatment strategies. Recent studies  have focused on characterizing the cellular and micro-structual origin of damage in rat medial collateral ligaments (MCLs). Provenzano et al.  demonstrated that structural damage occurs at 5.14% strain. In their study, damage was defined as the non-recoverable increase in strain after different stretches were applied to the MCLs.
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Investigation of Damage in Medial Collateral Ligaments
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Guo, Z, De Vita, R, Davis, FM, & Barrett, JG. "Investigation of Damage in Medial Collateral Ligaments." Proceedings of the ASME 2011 Summer Bioengineering Conference. ASME 2011 Summer Bioengineering Conference, Parts A and B. Farmington, Pennsylvania, USA. June 22–25, 2011. pp. 1275-1276. ASME. https://doi.org/10.1115/SBC2011-53335
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