Native tissues are composed of functional three-dimensional (3D) units on the scale of 100–1000μm. The 3D architecture of these repeating units underlies the coordination of multicellular processes such as proliferation, differentiation, migration and apoptosis. The requirement for 3D biomimetic matrices to mimic in vitro the ECM microarchitecture found in vivo becomes relevant in complex and vascularized tissue engineered models. Among others, photopolymerizable hydrogels offer tunable geometrical features similar to the macromolecular-based components of soft ECM , can be crosslinked either in vivo or in vitro in the presence of a photoinitiator agent (PI) using visible or ultraviolet (UV) light irradiation, and have shown good compatibility with several protocols for cell embedding at different size-scales. In the present study, a new protocol to obtain cell-laden hydrogel micropatterns with highly controlled geometrical features is presented, based on the combination of polydimethylsiloxane (PDMS) replica molding and UV photopolimerization of methacrylate gelatin (GelMA).
- Bioengineering Division
Validation of a Novel Microscale Mold Patterning Protocol Based on Gelatin Methacrylate Photopolymerizable Hydrogels
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Occhetta, P, Sadr, N, Piraino, F, Redaelli, A, Moretti, M, & Rasponi, M. "Validation of a Novel Microscale Mold Patterning Protocol Based on Gelatin Methacrylate Photopolymerizable Hydrogels." Proceedings of the ASME 2012 Summer Bioengineering Conference. ASME 2012 Summer Bioengineering Conference, Parts A and B. Fajardo, Puerto Rico, USA. June 20–23, 2012. pp. 1217-1218. ASME. https://doi.org/10.1115/SBC2012-80723
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