Cell alignment by underlying topographical cues has been shown to affect important biological processes such as differentiation and functional maturation in vitro. However, the routine use of cell culture substrates with micro/nano-topographies is currently hampered by the high cost and specialized facilities required to produce these substrates. Here we present commercially available optical media as substrates for aligning cells in culture. These optical media, including CD-R, DVD-R and optical grating, allow different cell types to attach, align and grow on them. This cytoskeletal reorganization enhanced the differentiation of cardiac (H9C2), and skeletal muscle (C2C12) cell lines. These low-cost commercially available optical media can serve as scalable substrates for research or drug safety screening applications.
- Bioengineering Division
Gratings on a Dish: A Scalable Cell Alignment Substrate on Optical Media
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Anene-Nzelu, CG, Choudhury, D, Li, H, Toh, Y, Ng, GSH, Liang, LH, & Yu, H. "Gratings on a Dish: A Scalable Cell Alignment Substrate on Optical Media." Proceedings of the ASME 2013 Summer Bioengineering Conference. Volume 1B: Extremity; Fluid Mechanics; Gait; Growth, Remodeling, and Repair; Heart Valves; Injury Biomechanics; Mechanotransduction and Sub-Cellular Biophysics; MultiScale Biotransport; Muscle, Tendon and Ligament; Musculoskeletal Devices; Multiscale Mechanics; Thermal Medicine; Ocular Biomechanics; Pediatric Hemodynamics; Pericellular Phenomena; Tissue Mechanics; Biotransport Design and Devices; Spine; Stent Device Hemodynamics; Vascular Solid Mechanics; Student Paper and Design Competitions. Sunriver, Oregon, USA. June 26–29, 2013. V01BT41A001. ASME. https://doi.org/10.1115/SBC2013-14109
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