Molecular transport of osteoarthritis (OA) therapeutics within articular cartilage is influenced by many factors, such as solute charge, that have yet to be fully understood. This study characterizes how solute charge influences local diffusion and convective transport of antibodies within the heterogeneous cartilage matrix. Three fluorescently tagged solutes of varying isoelectric point (pI) (4.7–5.9) were tested in either cyclic or passive cartilage loading conditions. In each case, local diffusivities were calculated based on local fluorescence in the cartilage sample, as observed by confocal microscopy. In agreement with past research, local solute diffusivities within the heterogeneous cartilage matrix were highest around 200–275 μm from the articular surface, but 3–4 times lower at the articular surface and in the deeper zones of the tissue. Transport of all 150 kDa solutes was significantly increased by the application of mechanical loading at 1 Hz, but local transport enhancement was not significantly affected by changes in solute isoelectric point. More positively charged solutes (higher pI) had significantly higher local diffusivities 200–275 μm from the tissue surface, but no other differences were observed. This implies that there are certain regions of cartilage that are more sensitive to changes in solute charge than others, which could be useful for future development of OA therapeutics.
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January 2019
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The Effect of Charge and Mechanical Loading on Antibody Diffusion Through the Articular Surface of Cartilage
Chris D. DiDomenico,
Chris D. DiDomenico
Meinig School of Biomedical Engineering,
Cornell University,
Ithaca, NY 14853
Cornell University,
Ithaca, NY 14853
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Lawrence J. Bonassar
Lawrence J. Bonassar
Professor
Meinig School of Biomedical Engineering,
Sibley School of Mechanical and Aerospace Engineering,
Cornell University,
149 Weill Hall,
Ithaca, NY 14853
email: lb244@cornell.edu
Meinig School of Biomedical Engineering,
Sibley School of Mechanical and Aerospace Engineering,
Cornell University,
149 Weill Hall,
Ithaca, NY 14853
email: lb244@cornell.edu
Search for other works by this author on:
Chris D. DiDomenico
Meinig School of Biomedical Engineering,
Cornell University,
Ithaca, NY 14853
Cornell University,
Ithaca, NY 14853
Lawrence J. Bonassar
Professor
Meinig School of Biomedical Engineering,
Sibley School of Mechanical and Aerospace Engineering,
Cornell University,
149 Weill Hall,
Ithaca, NY 14853
email: lb244@cornell.edu
Meinig School of Biomedical Engineering,
Sibley School of Mechanical and Aerospace Engineering,
Cornell University,
149 Weill Hall,
Ithaca, NY 14853
email: lb244@cornell.edu
1Corresponding author.
Manuscript received April 17, 2018; final manuscript received October 3, 2018; published online November 19, 2018. Assoc. Editor: David Corr.
J Biomech Eng. Jan 2019, 141(1): 014502 (5 pages)
Published Online: November 19, 2018
Article history
Received:
April 17, 2018
Revised:
October 3, 2018
Citation
DiDomenico, C. D., and Bonassar, L. J. (November 19, 2018). "The Effect of Charge and Mechanical Loading on Antibody Diffusion Through the Articular Surface of Cartilage." ASME. J Biomech Eng. January 2019; 141(1): 014502. https://doi.org/10.1115/1.4041768
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